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Pulse-chase labeling of rRNA synthesis of Nop8p-depleted cells. Pulse-chase labeling with [methyl-3H]methionine was performed with W303-1a (NOP8) and DG456 (GAL::NOP8) shifted from SCGal to SCGlu lacking methionine for 18 h. RNA samples were collected every 2 min. Total RNA (OD = 0.9) was loaded in each lane. See Materials and Methods for details.
