FIG. 7.

Analysis of pre-rRNA steady-state levels in Nop8p-depleted cells. RNA was analyzed from strain W303-1a (NOP8) at time zero and 28 h after transfer to YPD and from strain DG456 (GAL::NOP8) at time zero and 6, 12, 20, and 28 h after transfer to YPD. Equivalent amounts of total RNA were loaded in each lane. (A) Probe A, complementary to sequences upstream of site A₀ in the 5′ ETS; (B) probe B, complementary to a region downstream of the 18S rRNA 3′ end and upstream of site A₂ in ITS1; (C) probe C, complementary to sequences between sites A₂ and A₃ in ITS1; (D) probe D, complementary to sequences between sites C₁ and C₂ in ITS2. See Materials and Methods and Fig. 1 for details.