Fig. 4. Mutations on M lysine 14 induce apoptosis and are present in circulating stains of SARS-CoV-2.

a, b A549 WT or TRIM7 KO transfected with 500 ng of M-WT, M-K14R, or M-KallR mutants for 24 h and then stained with Apotracker Green, a representative dot blot. b Frequency of Apotracker⁺ cells, representative data of two independent experiments and each with two biological replicates. c–e M lysine mutations in SARS-CoV-2 sequences of circulating variants. Percentage of occurrence of M-K14 mutation across the clades (c) and membrane protein K14 mutation occurrence (d), or membrane protein K15 mutation occurrence (e). Red highlighted nodes indicate at least one mutation occurrence in the specific clade. Data were depicted as Mean ± SEM. Source data are provided in the Source data file.