Fig. 2.

TRPM7 was increased in SE models and participated in pyroptosis in vitro. A, B The representative protein bands and analysis of TRPM7 in hippocampus of PILO-treated C57BL/6 J mice (n = 6). C, D The representative protein bands and analysis of TRPM7 of cultured neurons treated with PILO for 24 h or cultured in Mg²⁺-free extracellular fluid for 3 h before cultured in the original medium for 21 h (n = 6).E–G Immunofluorescence analysis of TRPM7 (green) and NeuN (red) expression in hippocampus of PILO-treated C57BL/6 J mice, including the CA1, CA3 and DG regions (20 × lens or 60 × lens). The arrows indicated positive co-localization neurons (n = 9). Scale bar: 100 μm. DAPI (blue) was used to label nucleus. H, I The representative protein bands and analysis of TRPM7 in PILO-treated N2a cells (n = 6). J, K The representative protein bands and analysis of NLRP3, GSDMD, and caspase-1 p20 in TRPM7-overexpressed N2a cells (n = 5 or 6). Full scans of all the blots are in the Supplementary Note. *P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001