FIGURE 3.

Predominance of ether phospholipids over diacyl phospholipids in the SLS‐derived cells. The predominance of ether phospholipids over diacyl phospholipids was evident in the iPSCs and oligodendrocytes, and a similar finding was noted in the neurospheres. Comparisons between the control (WD39, 201B7, 119‐4F1, 119‐4F2) and SLS cells (1SLS12, 1SLS20, 1SLS35, 2SLS01, 2SLS02, 2SLS04) are presented as volcano plots, where the horizontal axis indicates the log intensity ratio of the percent relative abundance between SLS‐derived cells and control‐derived cells (i.e., control versus SLS), with the vertical axis indicating statistical significance (p‐values with a threshold of 0.05). Each circle represents one lipid species. Red circles indicate ether phospholipids and gray circles indicate diacyl phospholipids. Heatmap of the Z‐scores of the percent relative abundance of each phospholipid. The four columns on the left show the findings for cells obtained from 4 normal individuals and the six columns on the right show the results for the cells obtained from the 2 patients with SLS, SLS patient 1 and SLS patient 2. The listed lipid species in the heatmaps were selected based on their low ‐log₁₀ (p‐value) (more than 1) when constructing the volcano plot. The cells of the heatmap are colored according to the row z‐score value of the percent relative abundance obtained among 10 samples (4 from normal and 6 from SLS patients). Left, 4 columns (normal control), and right, 6 columns (SLS samples), are ordered within each group according to the clustering method using the row z‐scores. The volcano plots and heatmap were prepared for iPSCs (A), neurospheres (B), and oligospheres (C).