TABLE 2.
Antibodies used for Western blotting and immunofluorescence.
| Antibody | Host | Source | Catalog number | Dilution for staining | Dilution for Western blot |
|---|---|---|---|---|---|
| CD86 | Rabbit polyclonal | Huabio, China | ET1702-04 | 1:200 | 1:500 |
| anti-Mannose | Rabbit polyclonal | Abcam, United Kingdom | ab125028 | 1:1,000 | |
| P-JAK2 | Rabbit polyclonal | Huabio, China | ET1607-34 | 1:500 | |
| JAK2 | Rabbit polyclonal | Huabio, China | ET1607-35 | 1:500 | |
| STAT3 | Rabbit polyclonal | Cell Signaling Technology, United States | 12,640 | 1:1,000 | |
| P-STAT3 | Rabbit polyclonal | Cell Signaling Technology, United States | 9,145 | 1:1,000 | |
| Bcl-2 | Rabbit polyclonal | Huabio, China | ET1602-53 | 1:500 | |
| BAX | Rabbit polyclonal | Huabio, China | ET1603-34 | 1:10,000 | |
| Caspase | Rabbit polyclonal | Abcam, United Kingdom | ab184787 | 1:1,000 | |
| β-actin | Rabbit polyclonal | Cell Signaling Technology, United States | 4970S | 1:1,000 | |
| IgG-HRP | Mouse monoclonal | Thermo Fisher Scientific, United States | 7076S | 1:1,000 | |
| IgG-HRP | Rabbit polyclonal | Thermo Fisher Scientific, United States | 7074S | 1:1,000 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-31572 | 1:200 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-21202 | 1:200 | |
| F4/80 | Rabbit polyclonal | Huabio, China | RT1212 | 1:200 |