Fig. 4.

BRAF^V600E, rather than BRAF inhibitors, disrupted hematopoiesis and erythropoiesis. a Schematic diagram of lentiviral transduction and culture strategy of primary human UCB-CD34⁺ HSPCs. b Representative images of 300 HSPCs pre-cultured for 2 days in erythroid differentiation medium before seeded into Methocult H4435. c Corresponding colony counts and lineage ratios calculated on Day 14 of panel (b). Scale bar = 10 mm. d Flow cytometry analysis of HSPC surface markers on Day 12 in HSPC retention medium (top), and erythroid cell surface markers on Day 14 in erythroid differentiation medium (bottom), following lentiviral transduction or drug treatment. The control (Ctrl) and GDC groups were transduced with MSCV-GFP empty vector and treated with DMSO or 2 μM GDC-0879, respectively