Fig. 9.

(a) The incubating and treating procedure for detecting SA in Nicotiana glutinosa L. callus. (b) Fluorescence images of Nicotiana glutinosa L. callus cells incubated with probe 1 (10 μM) for 2, 4, 6, 8, and 10 h, followed by further incubation with SA (50 μM) for 30 min, showed time-dependent changes. The 1st column showed the control group, and the 2nd column showed the Nicotiana glutinosa L. callus cells incubated in probe 1 (10 μM) for 10 h. From top to bottom: bright field, red channel, and merged images. Scale bar: 100 μm. (c) The bar graphs depict the mean intensities of two-photon excited fluorescence in the callus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)