FIGURE 6.

LMAN2 promotes cisplatin resistance in BC by activating the MAPK pathway. (A) Venn diagram showing the number of overlapping genes between the three datasets as indicated. (B) Pearson's correlation analysis between LMAN2 expression and multiple proteins of the MAPK family in BC patients using the GEPIA database. (C) IP assays and Western blotting were conducted to determine the relationship between LMAN2 and MAPK9. (D) Intracellular localization analysis of LMAN2 and MAPK9 by IF assay. The intracellular localization of LMAN2 (red) and MAPK9 (green) is shown. Nuclei (blue) were stained with DAPI. (E) Western blot analysis of the protein levels of MAPK9 and downstream factors of the MAPK signaling pathway. (F) Flow cytometry analysis of the apoptosis of the transfected BC cells in the different treatment groups.