Figure 3.

Persister cell phenotype is shaped by genetic alterations present at diagnosis. A, Heatmap of log₂ copy ratio of chromosomes 1, 2, 3, 4, 7, 11, and 17 for 11 matched pairs with malignant cells percentage at diagnosis and definitive surgery sufficient to identify reliable copy number. Red, gain; blue, loss. B, Connected pairs plot showing the mutational burden, measured as the number of mutations per Mb, in 17 matched pairs at diagnosis and definitive surgery. C, Heatmap of median percent persister subtype fraction as a function of genetic alterations at diagnosis. del - deletion, NA - Non-Amplified. D, Bar plots of persister subtypes percentages for the CHOP and HTAPP cohorts in MYCN-amplified tumors (left) and -non-amplified tumors (right). Plots do not include tumors with MYCN amplification and TP53/ALK (N = 3, CHOP cohort). A, Amplified; NA, non-amplified; del, deletion; D, diagnosis; DS, definitive surgery; HTAPP, Human Tumor Atlas Pilot Project; ns, not significant.