TABLE 2.
Secondary metabolites identified in the Dichloromethane (VD-DCM) and Ethyl Acetate (VD-EA) leaf Extracts of Vitex doniana.
| Extract | Class | Name | Molecular formula | Molecular weight | Retention time (minutes) | % area |
|---|---|---|---|---|---|---|
| VD-DCM | Diterpenoid | Neophytadiene | C20H38 | 278 | 16.719 | 1.18 |
| Diterpenoid | Phytol, acetate | C22H42O2 | 338 | 19.637 | 0.51 | |
| Steroid | 9,19-Cyclocholestan-3-ol, 14-methyl-, (3β,5α)- | C28H48O | 400 | 22.919 | 2.50 | |
| Phytosterol | Stigmasta-4,22-dien-3β-ol | C29H48O | 412 | 23.638 | 2.96 | |
| Phytosterol | γ-Sitosterol | C29H50O | 414 | 25.864 | 41.75 | |
| Fatty acid ester | 6-Fluoro-2-trifluoromethylbenzoic acid, eicosyl ester | C28H44F4O2 | 488 | 26.020 | 3.81 | |
| Triterpenoid | 9,19-Cyclolanost-24-en-3-ol, (3β)- | C30H50O | 426 | 27.940 | 9.17 | |
| Triterpenoid | Lup-20(29)-en-3-one | C30H48O | 424 | 28.619 | 6.02 | |
| Phytosterol | Stigmasta-3,5-dien-7-one | C29H46O | 410 | 29.834 | 21.19 | |
| Phytosterol | Stigmast-4-en-3-one | C29H48O | 412 | 31.589 | 10.91 | |
| VD-EA | Sesquiterpenoid | (3R,3aR,3bR,4S,7R,7aR)-4-Isopropyl-3,7-dimethyloctahydro-1H-cyclopenta [1,3] cyclopropa[1,2]benzen-3-ol | C15H26O | 222 | 11.954 | 0.99 |
| Sesquiterpene | α-Copaene | C15H24 | 204 | 12.219 | 1.53 | |
| Sesquiterpene | Ylangene | C15H24 | 204 | 13.061 | 0.68 | |
| Sesquiterpene | Bicyclo[4.4.0]dec-1-ene, 2-isopropyl-5-methyl-9-methylene- | C15H24 | 204 | 13.863 | 2.46 | |
| Sesquiterpene alcohol | τ-Muurolol | C15H26O | 222 | 14.044 | 1.38 | |
| Sesquiterpene | α-Calacorene | C15H20 | 200 | 14.194 | 0.29 | |
| Sesquiterpene alcohol | 1-((1S,3aR,4R,7S,7aS)-4-Hydroxy-7-isopropyl-4-methyloctahydro-1H-inden-1-yl) e | C15H26O2 | 238 | 15.061 | 14.67 | |
| Sesquiterpene alcohol | (−)-Spathulenol | C15H24O | 220 | 15.189 | 1.19 | |
| Sesquiterpene | (3S,6S)-6-Isopropyl-3-methyl-2-(propan-2-ylidene)-3-vinylcyclohexanone | C15H24O | 220 | 15.650 | 2.08 | |
| Diterpenoid | Neophytadiene | C20H38 | 278 | 15.721 | 2.57 | |
| Sesquiterpene | Isoaromadendrene epoxide | C15H24O | 220 | 15.839 | 4.23 | |
| Aldehyde | 6-Nonenal, 3,7-dimethyl- | C11H20O | 168 | 15.908 | 2.39 | |
| Steroid | Pregn-4-ene-1,20-dione, 12-hydroxy-16,17-dimethyl- | C23H34O3 | 358 | 16.165 | 4.19 | |
| Phthalate ester | Phthalic acid, 4,4-dimethylpent-2-yl isobutyl ester | C19H28O4 | 320 | 16.260 | 1.18 | |
| Spiroketone | Spiro [2.5] octane, 5,5-dimethyl-4-(3-oxobutyl)- | C14H24O | 208 | 16.677 | 0.74 | |
| Acetic acid ester | Acetic acid, 3-(2,2-dimethyl-6-methylene-cyclohexylidene)-1-methyl-butyl ester | C16H26O2 | 250 | 16.876 | 1.40 | |
| Spiroketone | Spiro [2.5] octane, 5,5-dimethyl-4-(3-oxobutyl)- | C14H24O | 208 | 17.150 | 0.58 | |
| Tricyclic diterpenoid | 4,6,10,10-Tetramethyl-5-oxatricyclo [4.4.0.0(1,4)] dec-2-en-7-ol | C13H20O2 | 208 | 17.204 | 1.61 | |
| Kaurane diterpenoid | Kauran-18-aL, 17-(acetyloxy)-, (4β)- | C22H34O3 | 346 | 17.764 | 1.94 | |
| Triterpenoid | 3-O-Acetyl-6-methoxy-cycloartenol | C33H54O3 | 498 | 18.289 | 2.54 | |
| Diterpenoid | 3,7,11,15-Tetramethyl-2-hexadecen-1-ol | C20H40O | 296 | 18.634 | 2.95 | |
| Alkane | Tridecane, 5-propyl- | C16H34 | 226 | 21.974 | 0.60 | |
| Alkane | Hentriacontane | C31H64 | 436 | 23.800 | 11.61 | |
| Triterpene | Squalene | C30H50 | 410 | 25.231 | 1.24 | |
| Alkane | Pentatriacontane | C35H72 | 492 | 26.266 | 15.40 | |
| Flavonoid | 2-(3,4-Dimethoxyphenyl)-7-hydroxy-3-methoxy-4H-chromen-4-one | C18H16O6 | 328 | 28.975 | 2.36 | |
| Alkane | Tetratetracontane | C44H90 | 618 | 29.893 | 17.17 |