FIG. 8.

trans-Acting encapsidation by pSVRDM of HIV-2 vectors containing various amounts of the gag ORF. (A) Representative RPA using the KS2ES riboprobe and cytoplasmic and virion RNAs from COS-1 cells transfected with equal amounts of pSVRDM and vector. Lanes: S, RNA size markers (Century Plus); I, input probe diluted 1/100; Y, yeast RNA plus RNase; M, RNA from mock-transfected cells; 1, pSVRDM; 2, pSVRDM plus pSVRΔX; 3, pSVRDM plus pSVRΔAX; 4, pSVRDM plus pSVRΔHX; 5, pSVRDM plus pSVRΔpol; 6, pSVRDM plus pSVRΔHΔpol; 7, pSVRDM plus pSVRΔpolncm. (B) Quantification of vector encapsidation efficiencies, relative to that of the DM helper, in the experiments detailed above. Results are averages of at least two separate experiments; error bars represent the standard error of the mean between experiments. Numbering is as described for panel A.