Figure 2.

DIPY inhibits ferroptosis in LPS-induced ARDS mouse model. (A) Scheme of the experimental procedure for the LPS-induced ARDS mouse model. DIPY treatment (10 mg/kg, intraperitoneal) was administered 1 hour before the LPS challenge (low dose: 2.5 mg/kg; lethal dose: 20 mg/kg). After 24 hours, the BALF and the lungs of the mice (treated with low-dose LPS) were collected and analyzed. (B) Percent survival after administering lethal doses of LPS instillation is shown (n = 12 per group). (C-J) Low-dose LPS-challenged ARDS mice were pretreated with or without DIPY (n = 6 per group). Representative CT images of mouse lungs (C). Representative images of H&E-stained lung sections, with black arrows indicating infiltrating inflammatory cells, interstitial edema, and alveolar wall thickening (D). Scale bar, 50 μm. Lung injury scores (E). Measurements of lung wet/dry ratio and total protein in BALF (F). Total cell counts and neutrophil percentage in BALF (G). TNF-α and IL-1β expressions in BALF were measured by ELISA (H). MDA contents (upper panel) and Fe²⁺ levels (lower panel) in mouse lung tissues (I). Representative TEM images illustrating mitochondria in the alveolar epithelium, vascular endothelium, and airway epithelium, with black arrows indicating mitochondria (J). Scale bar, 500 nm. Results are shown as mean ± SD. Statistical significance is indicated as *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.