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. 2001 Dec;75(24):12088–12097. doi: 10.1128/JVI.75.24.12088-12097.2001

FIG. 2.

FIG. 2

In vitro expression studies following transient transfection of NIH 3T3 cells with the DNA-encoding E1 mutants. (A) Western blot. (B) Quantitative determination of intracellular expression of E1 mutants by capture ELISA. (C/) Immunoprecipitation (see Materials and Methods). The figure shows representative experiments. HCV proteins are indicated (E1* refers to the deglycosylated forms of E1), and positions of protein size markers are shown.