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. Author manuscript; available in PMC: 2024 Dec 2.
Published in final edited form as: Gene Ther. 2018 Aug 2;25(6):402–414. doi: 10.1038/s41434-018-0035-6

Fig. 2.

Fig. 2

PRT tear production assay and vector shedding analysis. Tear production was measured by PRT test and data are presented as the length of wetted phenol red thread from each eye (a). To detect the vector shedded in tear, tear samples were collected at 1 week (b, upper panel) and 4 weeks (b, lower panel) after subconjunctival injection then subjected to two rounds of PCR using vector-specific primers sets and detected by a GFP-specific probe via Southern blotting. Viral vector genomes plus host cell gDNA was used as positive control template. The numbers 1–6 represent the individual tear samples (six eyes per group)