TABLE 1.

Flow cytometry analysis of total pulmonary CD4⁺ cells isolated from mice mock infected or infected with rRSV/6120/mGM-CSF or rRSV/6120^a

Day	rRSV/6120/mGM-CSF				rRSV/6120				Mock
	PMCb	CD4+			PMC	CD4+			PMC	CD4+
		Totalc	IFN-γ+d	IL-4+d		Total	IFN-γ+	IL-4+		Total	IFN-γ+	IL-4+
5	151.2 ± 0.08*	79.08 ± 2.00*	2.44 ± 0.34†	1.58 ± 0.08*	55.60 ± 0.15	17.55 ± 1.73	0.57 ± 0.06	0.16 ± 0.02	12.0	2.51 ± 0.49	0.03 ± 0.00	0.02 ± 0.00
10	204.7 ± 16.99	86.24 ± 8.49	9.21 ± 1.16‡	0.93 ± 0.15§	265.6 ± 65.5	80.84 ± 17.33	5.40 ± 1.35	0.44 ± 0.09	61.0 ± 11	18.05 ± 3.55	0.23 ± 0.05	0.08 ± 0.02
32	119.5 ± 21.1	56.86 ± 8.26	4.11 ± 0.37	1.60 ± 0.58	105.5 ± 0.60	50.27 ± 5.62	3.35 ± 0.36	1.36 ± 0.14	57.0	11.97	0.31	0.17
38	47.5 ± 7.8	20.24 ± 2.82	4.93 ± 0.67	0.20 ± 0.04	51.0 ± 4.0	21.73 ± 1.35	5.48 ± 0.41	0.16 ± 0.01	77.0 ± 20.0	18.28 ± 4.03	1.85 ± 0.41	0.36 ± 0.09

^aMice were infected intranasally on day 0 with 10⁶ PFU of the indicated virus per animal or were mock infected. Animals from each group were harvested on days 5 and 10. The remaining animals were challenged on day 28 with 10⁶ PFU of wt RSV per animal and sacrificed on days 32 and 38. The average number of isolated PMC was calculated. Total PMC were analyzed by flow cytometry with immunostaining for CD4, IFN-γ, and IL-4. Four animals per group per day were used except for the mock group, for which two, two, one, and three animals were used on days 5, 10, 32, and 38, respectively. Statistical significance was calculated by Student's t test compared to the rRSV/6120 control: *, P < 0.001; †, P < 0.01; ‡, P < 0.1; §, P < 0.05. 

^bMean number of total PMC per mouse (in units of 100,000) with SE. 

^cMean number of pulmonary CD4⁺ cells per mouse (in units of 100,000) with SE. 

^dMean number of CD4⁺ lymphocytes positive for IFN-γ or IL-4 following in vitro stimulation (in units of 100,000) with SE.