FIG. 8.

TEV IRES RNA inhibits translation when present in trans. Unfractionated wheat germ lysate was programmed with uncapped TEV-luc-A₅₀ (A) or uncapped Con₁₄₄-luc-A₅₀ (B) mRNAs at a concentration of 0.67 ng/μl. TEV IRES RNA was added in trans to each lysate at the indicated molar ratio, and the degree to which each mRNA was translated was determined by luciferase assays. Luciferase activity is indicated as the average (from 2 μl of lysate) of three translation reactions, with the standard deviation for each construct shown. The degree to which an mRNA was translated relative to expression from the mRNA in lysate containing no TEV IRES RNA is indicated as a percentage.