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Estimation of d-glucose (A), d-galactose (B), and d-mannose (C) utilization by S. cerevisiae EBY.VW4000 harboring WT Xltr1p and its variants Y301W, Q291A, L174A, and N297A. All experiments were performed using three biological replicates, with the strain harboring the plasmid pJFE3 without Xltr1p representing the negative control.
