Fig. 7.

Structural analyses of the Y301W and Q291A mutants of Xltr1p. (A) Substrate docking analysis of Xltr1p (WT or Y301W) with d-galactose. a. Overall structures of WT (gray) and Y301W (green) Xltr1p with docked d-galactose, b. Vertical view, and c. Front view, of Y301 and W301 residues (before [in red] and after [in blue] mutation, respectively). (B) Schematic representation of the interaction between the W301 residue of Xltr1p Y301W and d-glucose (a), d-mannose (b), or d-galactose (c). (C) Predictions of transmembrane helices using PHYRE2. Predictions using DeepTMHMM are shown in Fig. S4. (D) Schematic representation of the interactions between Q291 of WT Xltr1p (a) or A291 of Q291A (b) with d-mannose.