Fig 4. OsHIPP20 is a susceptibility gene (S-gene).

(A) Results of punch inoculation of conidia of a compatible isolate Sasa2 to the wild-type Sasanishiki (WT) and T2 generation of two homozygous KO rice groups (guide RNA-1 [g1] and guide RNA-2 [g2], each with two replicates [g1-1, g1-2 and g2-1 and g2-2]). Box plots show lesion area sizes in the rice lines (top). Statistical significance is shown after Wilcoxon rank sum test. Photos of typical lesions developed on the leaves after inoculation of M. oryzae (bottom). The number (N) of leaves used for experiments are indicated below. (B) Results of punch inoculation of Sasa2 conidia to T2 progeny segregated to the wild type allele (W/W) and KO-type allele (m/m) from a T1 heterozygous KO line (g1-3). Statistical significance is shown according to Wilcoxon rank sum test. (C) Results of spray inoculation of conidia of a compatible isolate Ken53-33 to the wild-type Sasanishiki (WT) and T2 generation of the OsHIPP20-knockout lines g1-2 and g2-1. Box plots show the relative content of fungal actin gene DNA (M. oryzae actin gene DNA / rice actin gene DNA) as determined by quantitative PCR. Statistical significance is shown according to Wilcoxon rank sum test. (D) No growth defect in OsHIPP20 KO line as compared to the wild type one month after seed sowing. Top: Overview of the W/W and m/m plants. Bottom: Box plot showing plant height distribution of W/W and m/m plants segregated from the g1-3 heterozygous KO line. Statistical significance is shown according to Wilcoxon rank sum test. Scale bar: 5cm.