Figure 5. Cytokine secretion by AM is more reliant on glycolysis than MDM.

Human AM (A, C, E) isolated from bronchoalveolar lavage fluid. PBMC were isolated from buffy coats and MDM (B, D, F) were differentiated and adherence purified for 7 days in 10% human serum. Cells were left unprimed (black) or primed with IFN-γ (red) or IL-4 (blue; both 10 ng/ml) for 24 hr. Cells were left untreated (solid) treated with 2DG (5 mM; empty) for 1 hr prior to stimulation with iH37Rv (MOI 1–10; square) or LPS (100 ng/ml; triangle) or left unstimulated (circle). Supernatants were harvested 24 hr after stimulation and concentrations of IL-1β (A, B), TNF (C, D) and IL-10 (E, F) were quantified by ELISA. Each linked data point represents the average of technical duplicates for one individual biological donor (AM; n=12–13, MDM; n=8–10). Statistically significant differences were determined using two-way ANOVA with a Tukey post-test (A–D); *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001 or #p≤0.05, ##p≤0.01 (where IFN-γ primed data sets were excluded for post-test analysis to analyse statistical differences between no cytokine and IL-4 treated data sets).