Figure 7.

Effect of hsa_circ_0001314 on the proliferative and metastatic capacity of BC cells. (a) Fluorescence signals of the transfected cells were observed under a fluorescence microscope. (b) The siRNAs of hsa_circ_0001314 were transfected to BC cells. Compared with si-hsa_circ_0001314-1, si-hsa_circ_0001314-2 had higher interference efficiency (⁣^∗p < 0.050, ⁣^∗∗p < 0.010). (c) CCK-8 assay revealed that the knockdown of hsa_circ_001314 significantly reduced the proliferation of BC cells (⁣^∗∗∗∗p < 0.0001). (d) Wound healing assay revealed that the knockdown of hsa_circ_001314 dramatically inhibited the migration of BC cells (⁣^∗∗p < 0.010, ⁣^∗∗∗∗p < 0.0001). (e) Transwell assay revealed that the knockdown of hsa_circ_001314 dramatically inhibited the invasion of BC cells (⁣^∗∗∗∗p < 0.0001). (f) Flow cytometry assay revealed that the knockdown of hsa_circ_001314 significantly induced cell apoptosis in BC cells (⁣^∗∗p < 0.010).