Figure 8.

hsa_circ_0001314 binds to hsa-miR-548aj-3p in BC and regulates the expression of MAPK8/MAP3K1. (a) RT-qPCR revealed that the level of hsa-miR-548aj-3p in BC tissues was lower than that in adjacent tissues (⁣^∗∗∗∗p < 0.0001). (b) Correlation analysis indicated that the expression levels of hsa_circ_0001314 and hsa-miR-548aj-3p were negatively correlated in BC tissues (r = −0.5750, p = 0.0249). (c) After the knockdown of hsa_circ_0001314, the expression level of hsa-miR-548aj-3p was higher than that in the NC (⁣^∗∗p < 0.010). (d) Transfection efficiency was verified after transfection of hsa-miR-548aj-3p mimics in MCF-7 (⁣^∗∗p < 0.010). (e) After overexpression of hsa-miR-548aj-3p, the expression level of hsa_circ_0001314 was lower than that of NC (⁣^∗p < 0.050). (f) Compared with the expression in MCF-7, the expression of MAPK8 and MAP3K1 genes was significantly reduced after knockdown hsa_circ_0001314, and there was no significant change in KRAS (⁣^∗p < 0.050, ⁣^∗∗∗∗p < 0.0001; the ns indicates that the difference is not significant).