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Optimal concentration and time of action of N-acetylcysteine (NAC) for immunofluorescence screening. (a and b) Changes in fluorescence intensity of GFAP and CX43 in each group at 24 hr of NAC treatment. NC, normal control group; lipopolysaccharide (LPS), 1 μg/mL LPS; NAC1, LPS + 5 mM; NAC2, LPS + 2.5 mM; NAC3, LPS + 1 mM; and NAC4, LPS + 0.5 mM. Scale bar = 50 μm; n = 3. ∗P < 0.05 vs. NC group. #P < 0.05 vs. LPS group.
