Figure 4.

MiR-1292-5p inhibits FBLN2 expression in HCC by directly targeting its 3′-UTR. (A) MiR-1292-5p and the 3′-UTR sequences of FBLN2 were complementary to each other according to the predictions using the TargetScan algorithm. (B) SMMC7721/SOR and Huh7/SOR cells were treated with GV657-FBLN2 vector, followed by the transfection with biotinylated miR-1292-5p mimics or miR-NC.The binding RNAs were checked using Real-time PCR. **P<0.01 versus miR-NC group. (C) SMMC7721/SOR and Huh7/SOR cells were co-transfected with reporter construct and miR-1292-5p mimics and cel-miR-239b. Renilla luciferase activity of reporter constructs (psiCHECK-1) containing the wild-type and mutated FBLN2 3′UTR was evaluated 48 h after transfection. **P<0.01. (D and E) FBLN2 expression in HCC cells with the transfection of miR-1292-5p mimics was detected using Real-time PCR assay (D) and Western Blot assay (E). **P<0.01 versus Ctrl group. (F) and (G) FBLN2 expression in HCC cells with the transfection of miR-1292-5p inhibitor was detected using Real-time PCR assay (F) and Western Blot assay (G). **P<0.01 versus Ctrl group. For the Figure B, C, D and F, data are shown as means±SD of three independent experiments. For the Figure E and G, Data are shown as representatives (left) or means±SD (right) from three independent experiments.