Table 1:
Changes in sleep-wake architecture following NtsLH neuron lesions
| Number of bouts | Mean bout duration (s) | ||||||
|---|---|---|---|---|---|---|---|
| Control | Incomplete | Lesion | Control | Incomplete | Lesion | ||
| Wake | 24 h | 261.3 ± 17.1 | 320.9 ± 23.0 | 325.6 ± 22.8 | 166.3 ± 13.1 | 134.1 ± 14.6 | 114.9 ± 12.4†* |
| Light | 137.4 ± 12.7 | 169.4 ± 16.2 | 176.4 ± 6.1 | 101.1 ± 9.1 | 91.4 ±11.8 | 74.9 ± 4.5 | |
| Dark | 124.0 ± 8.0 | 146.1 ± 12.2 | 149.3 ± 17.4 | 211.0 ± 16.2 | 176.8 ± 29.9 | 144.3 ± 9.9 | |
| REM | 24 h | 79.5 ± 17.1 | 91.5 ± 11.9 | 95.1 ± 10.0 | 64.0 ± 2.7 | 67.2 ± 6.1 | 58.1 ± 0.6 |
| Light | 55.4 ± 8.3 | 56.9 ± 8.9 | 67.1 ± 7.4 | 63.8 ± 2.8 | 76.9 ± 11.1 | 56.4 ± 1.2 | |
| Dark | 24.1 ± 3.8 | 39.0 ± 9.7 | 28.0 ± 3.7 | 61.0 ± 4.4 | 57.5 ± 3.4 | 62.6 ± 1.8 | |
| NREM | 24 h | 268.8 ± 16.6 | 320.8 ± 22.9 | 326.0 ± 22.7 | 147.8 ± 9.9 | 133.7 ± 9.3 | 141.7 ± 11.7 |
| Light | 143.8 ± 12.7 | 182.5 ± 12.2 | 176.7 ± 6.1† | 161.5 ± 8.7 | 146.4 ± 15.5 | 149.3 ± 8.3 | |
| Dark | 125.8 ± 7.6 | 146.0 ± 12.2 | 150.0 ± 17.3 | 131.1 ± 16.2 | 121.0 ± 5.3 | 120.9 ± 11.4 | |
Bout number and average duration of individual sleep-wake states during light and dark periods and the entire 24-h day in mice with complete (>75% bilateral cell loss) or incomplete loss of NtsLH neurons and sham controls. All data are mean ± SEM.
†
P<0.05: One-way ANOVA,
*
P<0.05: post-hoc test by Tukey’s multiple comparison (for mean duration of wake in 24 h; F = 3.67, P = 0.044, control vs lesion: P = 0.038, for the number of bouts of NREM in the light period; F = 3.65, P = 0.045).