FIG. 1.

Mutational analysis of the acidic cluster region of UL11. (A) Inactivation of the wild-type sequence. The 96-amino-acid UL11 sequence (open rectangles) is shown with the position of its acidic cluster (AC) (solid black box). N-terminal myristylation (wavy line) is a natural modification of UL11 and was also provided by the first 10 amino acids of the Src oncoprotein (solid rectangle with wavy line). All constructs have the GFP protein (open oval) fused to their C termini. (B) Replacement with foreign acidic cluster sequences. The wild-type sequence of the UL11 acidic cluster is shown (residues 37 to 43). Wild-type and mutant acidic cluster sequences from furin and Nef were inserted in place of the UL11 sequence as shown. All constructs were expressed as UL11-GFP chimeras.