FIG. 8.

Membrane binding of UL11 derivatives. Transfected A7 cells were harvested and washed in NTE buffer and then allowed to swell for 1 h in hypotonic buffer. Cells were disrupted by Dounce homogenization, and nuclei were removed by a low-speed spin. To separate membrane-bound molecules from soluble forms, the supernatants were subjected to centrifugation at 100,000 × g. The soluble (S) and pellet (P) fractions were collected and analyzed for the presence of UL11 protein by fluorometry (A) or Western blot analysis (B). The fraction pelleted was calculated by dividing the amount pelleted by the total amount of protein (soluble plus pellet). Error bars indicate standard deviations.