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Immunounification of 35S-labeled virions by MAbs. SDS-PAGE and fluorography of labeled TGEV virions immunopurified with MAb 25.22 (A), MAb 9D.B4 (B), and MAb 3D.E3 (C) were performed in the presence of increasing amounts of cell lysates from BHK-pAPN cells expressing Mwt (top row), MΔ1–50 (second row), MΔ253–262 (third row), and MΔ146–262 (bottom row). vT7, lysate of vT7-infected BHK-pAPN cells.
