TABLE 2.
Peptide mass fingerprints from SDS-PAGE-purified TGEV structural proteinsa
| Protein | Predicted peptide | Exptl mass (mean ± SD)b |
|---|---|---|
| S | FYLTPR | 795.91 ± 0.714 |
| YVCNGNPR | 978.95 ± 0.714 | |
| DVQLTLFR | 991.05 ± 0.714 | |
| NLDDKFYLTPR | 1,381.39 ± 0.583 | |
| FCLSLSPVGANCK | 1,452.23 ± 0.714 | |
| LTALNAFVSQTLTR | 1,534.37 ± 0.714 | |
| EWPNIGGSWLEGLK | 1,585.32 ± 0.714 | |
| TFGLVVKDVQLTLFR | 1,735.52 ± 0.714 | |
| ISFENQWSGTVTFGDMR | 1,974.44 ± 0.707 | |
| LNVVVNGYPYSITVTTTR | 1,996.60 ± 0.714 | |
| TLLSGLYYTSLSGDLLGFK | 2,047.63 ± 0.714 | |
| LLTQYVSACQTIEQALAMGAR | 2,323.76 ± 0.714 | |
| LPPNSDVVLGDYFPTVQPWFNCIR | 2,834.05 ± 0.714 | |
| ATTLEVAGTLVDLWWFNPVYDVSYYR | 3,078.21 ± 0.707 | |
| MTMYTASLAGGITLGALGGGAVAIPFAVAVQAR | 3,135.33 ± 0.714 | |
| N | VSWGDESTK | 1,008.46 ± 0.007 |
| YHKPKDDPK | 1,112.54 ± 0.023 | |
| DGAMNKPTTLGSR | 1,363.61 ± 0.047 | |
| KDDSVEQAVLAALK | 1,486.56 ± 0.396 | |
| DDSVEQAVLAALKK | 1,486.56 ± 0.396 | |
| VPGEFQLEVNQSR | 1,502.72 ± 0.046 | |
| EDGDQIEVTFTHK | 1,518.66 ± 0.044 | |
| WFFYLGTGPHADAK | 1,772.78 ± 0.067 | |
| FDGKVPGEFQLEVNQSR | 1,949.89 ± 0.070 | |
| TGQFLQQINAYARPSEVAK | 2,121.03 ± 0.067 | |
| NNNIPLSFFNPITLQQGSK | 2,132.10 ± 0.015 | |
| M | SIQLYRR | 935.2 ± 0.575 |
| SDTDLSCR | 953.07 ± 0.595 | |
| AGDSTEAR | 969.09 ± 0.583 | |
| TIVYTLVGK | 993.26 ± 0.577 | |
| YVMVALPSR | 1,035.23 ± 0.583 | |
| AILCVSALGR | 1,059.27 ± 0.589 | |
| TIVYTLVGKK | 1,121.35 ± 0.571 | |
| IAGGMNIDNLPK | 1,242.31 ± 0.583 | |
| SWWSFNPETK | 1,281.25 ± 0.583 | |
| ASSATGWAYYVK | 1,302.95 ± 0.577 | |
| TKSWWSFNPETK | 1,510.39 ± 0.571 | |
| TDNLSEQEKLLHMV | 1,656.81 ± 0.015 | |
| SWWSFNPETKAILCVSALGP | 2,321.83 ± 0.577 | |
| SYVLPLEGVPTGVTLTLLSGNLYAEGFK | 2,938.21 ± 0.554 | |
| E | TVIIVPAQHAYDAYK | 1,688.86 ± 0.054 |
a
Boldface indicates the sequence and the mass of the C terminus of the M protein.
b
The mean was determined from the results of three independent experiments.