Table 1.

List of oligos used for cloning

Name	Sequence (5’ to 3’)	Purpose
ENG c.360＋1G>A_spacer_F	caccGTGGAGGGAACACACTCACGTgtttt	Golden Gate assembly cloning into pU6-tevopreq1-GG-acceptor plasmid
ENG c.360＋1G>A_spacer_R	ctctaaaacACGTGAGTGTGTTCCCTCCAC
epegRNA scaffold_F	AGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG
epegRNA scaffold_R	GCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAG
ENG c.360＋1G>A_extension_F	gtgcACTTGGCCTACaTGAGTGTGTTCCC
ENG c.360＋1G>A_extension_R	cgcgGGGAACACACTCAtGTAGGCCAAGT
hU6 promoter_F	GAGGGCCTATTTCCCATGATT	Sanger sequencing