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List of PCR primers for Sanger sequencing
| Name | Sequence (5’ to 3’) | Size (bp) | Purpose |
|---|---|---|---|
| ENG_e3_F | CTGCCTGTCTGGGTGGCACAACCT | 269 | gDNA PCR, Sanger sequencing |
| ENG_e3_R | CAGTAGGGACCTCCCATGGCCAGA | ||
| ENG OT(1)_F | GCAAACGCTGTCCCTATCCT | 262 | Off-target (1) Sanger sequencing |
| ENG OT(1)_R | CTCTCCCACCAACCTGGAAC | ||
| ENG OT(2)_F | CCCCAGAGAGGTGATCGAGA | 333 | Off-target (2) Sanger sequencing |
| ENG OT(2)_R | CATGGCAGGGTTTAGCCTCA |
gDNA PCR: genomic DNA polymerase chain reaction.
