Table 1.
List of oligonucleotides used for sgRNA cloning
| sgRNA | 5’-sequence-3’ | Usage |
|---|---|---|
| mUsp7-F | CACCGGAGTGATGGGCACAGCAACG | CRISPR/Cas9-based KO |
| mUsp7-R | AAACCGTTGCTGTGCCCATCACTCC | CRISPR/Cas9-based KO |
| mUsp12-F | CACCGTCGGCATTAGAGAAAGAGAT | CRISPR/Cas9-based KO |
| mUsp12-R | AAACATCTCTTTCTCTAATGCCGAC | CRISPR/Cas9-based KO |
| mUsp21-F | CACCGCTCAAGAAACTGGAGCTGGG | CRISPR/Cas9-based KO |
| mUsp21-R | AAACCCCAGCTCCAGTTTCTTGAGC | CRISPR/Cas9-based KO |
| mUsp37-F | CACCGGTAAGGATGCAGAGGAAATG | CRISPR/Cas9-based KO |
| mUsp37-R | AAACCATTTCCTCTGCATCCTTACC | CRISPR/Cas9-based KO |
sgRNA: single guide RNA, KO: knockout.