FIG. 3.

HIV-1 infections mediated by mutant CCR5 coreceptors. (A and B) Infection of the CCR5(Y14N) clonal panel by pseudotyped HIV-gpt virions bearing the wild-type envelope or the mutant JRCSF envelopes described in Table 2. Infections were performed and quantitated as described in Materials and Methods. Relative infectivities were determined by dividing the titer measured on a given cell clone by the titer determined on a HeLa-CD4 clone expressing high levels of wild-type CCR5 (clone JC.53). (C and D) Infection of the CCR5(G163R) clonal panel by the same pseudotyped viruses as in panels A and B. Data points represent means of four independent assays, and error bars represent the standard error of the mean. The CCR5(Y14N) and CCR5(G163R) clonal panels were described previously (32).