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. 2024 Nov 1;56(11):2423–2435. doi: 10.1038/s12276-024-01338-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Input voltage-clamp pulse protocol used for stimulating prestin in the outer hair cells. A 1 kHz sine wave with a 10 mV amplitude was simultaneously applied at −150 to 100 mV for a 250 ms duration. b, c Representative traces of Myh1^+/+ and Myh1^−/− mouse outer hair cell recordings at postnatal Day 21. d Voltage-normalized nonlinear capacitance (NLC) relationship of Myh1^+/+ (n = 6) and Myh1^−/− (n = 8) mouse outer hair cells. The bell-shaped curve was fit to the derivative of the Boltzmann equation. e V_h values calculated from the outer hair cells of Myh1^+/+ and Myh1^−/− mice. f Normalized charge transfer density calculated from the outer hair cells of Myh1^+/+ and Myh1^−/− mice. Data are presented as the means ± SEMs. Statistical comparisons were performed using two-sample independent t-tests in (e) and (f). *P < 0.05 and **P < 0.01.