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. 2024 Dec 2;15:10486. doi: 10.1038/s41467-024-54811-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — a Live imaging of sei1∆ cells expressing plasmid-based NLS-SEI1, the indicated mGFP-tagged constructs and genomically integrated NLS-PA-mCherry sensor. All mGFP constructs were expressed from the GPD promoter, except PET10 (PET10 promoter) and PEX30 (TPI1 promoter). nLD, nuclear lipid droplet; cLD, cytoplasmic lipid droplet; pER, peripheral endoplasmic reticulum. Scale bar, 2 μm. b Live imaging of sei1∆ cells expressing plasmid-based NLS-SEI1, NLS-PA-mCherry-VN and the indicated VC-tagged constructs. All VC-tagged constructs were expressed from the GPD promoter, except PET10 (ADH1 promoter) and PEX30 (TPI1 promoter). nLD, nuclear lipid droplet. Scale bar, 2 μm. c Summary of Sei1-formed nLD proteome and lipidome.