FIG. 2.

HCMV induced altered mRNA cellular mRNA levels in infected cells. Expression data for 1,425 genes judged as being significantly altered by HCMV were normalized by gene and clustered using the hierarchical clustering program CLUSTER. The clusters were then visualized using the TREEVIEW program (A). Putative clusters are noted by numbers to the right of the image. Elevated (red) and reduced (green) mRNA levels are indicated. Genes with functions relating to cell cycle, cell proliferation, or oncogenesis were identified by a Perl program that annotated Affymetrix GeneChip data with information about the biological function of the genes using the GeneOntology database. These genes were then clustered separately and visualized using TREEVIEW (B). Alpha-interferon-sensitive genes found to be modulated by HCMV were also clustered separately (C).