FIG. 3.
HCMV replication represses the up-regulation of cellular genes. HFFs were infected at 6 PFU/cell with purified AD169 virus particles. For UV-irradiated HCMV, virus samples were exposed to UV radiation sufficient to reduce infectivity by 105-fold (data not shown). Infections were carried out in duplicate, and at 6 hpi, the cells were lysed and RNA samples were extracted and analyzed by Affymetrix GeneChip analysis to determine the number of up-regulated or down-regulated genes. Genes that changed by threefold or greater and had a difference call of I, MI, D, or MD in both replicates were considered to be significantly regulated. The total number of genes up and down-regulated is shown. CHX-HCMV, HCMV treated with cycloheximide.