FIG. 2.

The MHC class I-associated protein is a glycoprotein. (A) HHV-7-infected SupT1 cells (72 h postinfection) were pulse-labeled for 10 min and then chased for the indicated times. W6/32 immune complexes were digested with or without Endo-H. Endo-H-resistant forms of MHC class I heavy chain (HC^r) and gp60 (gp60^r1 and gp60^r2) are indicated. (B) HHV-7-infected SupT1 cells were pulse-labeled for 10 min and then chased for the indicated times in the absence or presence of BFA and immunoprecipitated with W6/32. (C) HHV-7-infected SupT1 cells were pulse-labeled for 10 min and then chased for 40 min in the presence of BFA. W6/32 immune complexes were treated with increasing concentrations of Endo-H, as indicated. The estimated number of glycans added to gp60 are indicated on the right.