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. 1979 Aug 15;182(2):347–351. doi: 10.1042/bj1820347

Intracellular localization of long-chain acyl-coenzyme A hydrolase and acyl-L-carnitine hydrolase in brown adipose tissue from guinea pigs.

R K Berge, E Slinde, M Farstad
PMCID: PMC1161313  PMID: 41517

Abstract

The activities of long-chain acyl-CoA hydrolase (palmitoyl-CoA hydrolase, EC 3.1.2.2) and long-chain acyl-L-carnitine hydrolase, EC 3.1.1.28) in brown adipose tissue from cold-exposed and control guinea pigs were studied. Mitochondria from cold-exposed animals hydrolysed 21 nmol of palmitoyl-CoA/min per mg of protein and 1.3 nmol of palmitoyl-L-carnitine/min per mg of protein, and the specific activities were respectively 2 and 5 times as high in cold-exposed as in control animals. The subcellular-localization studies showed that both the long-chain acyl-CoA hydrolase and long-chain acyl-L-carnitine hydrolase were localized in the mitochondria. A location also in the soluble fraction cannot be excluded. The long-chain acyl-CoA hydrolase activity was doubled when the mitochondria were disrupted; this indicates that the enzyme is localized in the matrix compartment.

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Selected References

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  1. ARRIGONI O., SINGER T. P. Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases. Nature. 1962 Mar 31;193:1256–1258. doi: 10.1038/1931256a0. [DOI] [PubMed] [Google Scholar]
  2. Aas M. Organ and subcellular distribution of fatty acid activating enzymes in the rat. Biochim Biophys Acta. 1971 Feb 2;231(1):32–47. doi: 10.1016/0005-2760(71)90253-0. [DOI] [PubMed] [Google Scholar]
  3. Bar-Tana J., Rose G., Shapiro B. The purification and properties of microsomal palmitoyl-coenzyme A synthetase. Biochem J. 1971 Apr;122(3):353–362. doi: 10.1042/bj1220353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barden R. E., Cleland W. W. 1-Acylglycerol 3-phosphate acyltransferase from rat liver. J Biol Chem. 1969 Jul 10;244(13):3677–3684. [PubMed] [Google Scholar]
  5. Berge R. K., Farstad M. Dual localization of long-chain acyl-CoA hydrolase in rat liver: one in the microsomes and one in the mitochondrial matrix. Eur J Biochem. 1979 Mar 15;95(1):89–97. doi: 10.1111/j.1432-1033.1979.tb12942.x. [DOI] [PubMed] [Google Scholar]
  6. Bernson S. M. Acetyl-CoA hydrolase; activity, regulation and physiological significance of the enzyme in brown adipose tissue from hamster. Eur J Biochem. 1976 Aug 16;67(2):403–410. doi: 10.1111/j.1432-1033.1976.tb10705.x. [DOI] [PubMed] [Google Scholar]
  7. Brophy P. J., Vance D. E. The synthesis and hydrolysis of long-chain fatty acyl-coenzyme A thioesters by soluble and microsomal fractions from the brain of the developing rat. Biochem J. 1976 Nov 15;160(2):247–251. doi: 10.1042/bj1600247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cannon B., Sundin U., Romert L. Palmitoyl coenzyme A: a possible physiological regulator of nucleotide binding to brown adipose tissue mitochondria. FEBS Lett. 1977 Feb 15;74(1):43–46. doi: 10.1016/0014-5793(77)80748-5. [DOI] [PubMed] [Google Scholar]
  9. Colli W., Hinkle P. C., Pullman M. E. Characterization of the fatty acid elongation system in soluble extracts and membrane preparations of rat liver mitochondria. J Biol Chem. 1969 Dec 10;244(23):6432–6443. [PubMed] [Google Scholar]
  10. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Drahota Z., Alexandre A., Rossi C. R., Siliprandi N. Organization and regulation of fatty acid oxidation in mitochondria of brown adipose tissue. Biochim Biophys Acta. 1970 Jun 30;205(3):491–498. doi: 10.1016/0005-2728(70)90114-3. [DOI] [PubMed] [Google Scholar]
  13. Farstad M., Bremer J., Norum K. R. Long-chain acyl-CoA synthetase in rat liver. A new assay procedure for the enzyme, and studies on its intracellular localization. Biochim Biophys Acta. 1967 Mar 15;132(2):492–502. doi: 10.1016/0005-2744(67)90167-2. [DOI] [PubMed] [Google Scholar]
  14. Flatmark T., Pedersen J. I. Brown adipose tissue mitochondria. Biochim Biophys Acta. 1975 Mar 31;416(1):53–103. doi: 10.1016/0304-4173(75)90013-0. [DOI] [PubMed] [Google Scholar]
  15. Flatmark T., Ruzicka F. J., Beinert H. The pattern of iron--sulfur centers in brown adipose tissue mitochondria: preponderance of ETF dehydrogenase and invariance with the thermogenic state. FEBS Lett. 1976 Mar 15;63(1):51–55. doi: 10.1016/0014-5793(76)80192-5. [DOI] [PubMed] [Google Scholar]
  16. Heaton G. M., Nicholis D. G. Hamster brown-adipose-tissue mitochondria. The role of fatty acids in the control of the proton conductance of the inner membrane. Eur J Biochem. 1976 Aug 16;67(2):511–517. doi: 10.1111/j.1432-1033.1976.tb10717.x. [DOI] [PubMed] [Google Scholar]
  17. Hoppel C. L., Tomec R. J. Carnitine palmityltransferase. Location of two enzymatic activities in rat liver mitochondria. J Biol Chem. 1972 Feb 10;247(3):832–841. [PubMed] [Google Scholar]
  18. Jezyk P. F., Hughes H. N. Studies on the hydrolysis and utilization of long chain acyl CoA thioesters by liver microsomes. Lipids. 1971 Feb;6(2):107–112. doi: 10.1007/BF02531325. [DOI] [PubMed] [Google Scholar]
  19. Kurooka S., Hosoki K., Yoshimura Y. Some properties of long fatty acyl-coenzyme A thioesterase in rat organs. J Biochem. 1972 Apr;71(4):625–634. [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Mahadevan S., Sauer F. Carnitine ester hydrolase of rat liver. J Biol Chem. 1969 Aug 25;244(16):4448–4453. [PubMed] [Google Scholar]
  22. Nicholls D. G. The bioenergetics of brown adipose tissue mitochondria. FEBS Lett. 1976 Jan 15;61(2):103–110. doi: 10.1016/0014-5793(76)81014-9. [DOI] [PubMed] [Google Scholar]
  23. Normann P. T., Ingebretsen O. C., Flatmark T. On the rate-limiting step in the transfer of long-chain acyl groups across the inner membrane of brown adipose tissue mitochondria. Biochim Biophys Acta. 1978 Feb 9;501(2):286–295. doi: 10.1016/0005-2728(78)90034-8. [DOI] [PubMed] [Google Scholar]
  24. Pedersen J. I., Slinde E., Grynne B., Aas M. The intracellular localization of long-chain acyl-CoA synthetase in brown adipose tissue. Biochim Biophys Acta. 1975 Jul 22;398(1):191–203. doi: 10.1016/0005-2760(75)90182-4. [DOI] [PubMed] [Google Scholar]
  25. Prusiner S. Spectroscopic evidence for the control of respiration prior to phosphorylation in hamster brown fat cells. J Biol Chem. 1970 Jan 25;245(2):382–389. [PubMed] [Google Scholar]
  26. Slinde E., Morild E., Flatmark T. A general and rational approach to the optimal recovery of mitochondria by differential centrifugation in homogenous media. Anal Biochem. 1975 May 26;66(1):151–158. doi: 10.1016/0003-2697(75)90733-2. [DOI] [PubMed] [Google Scholar]
  27. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vollset S. E., Farstad M. A study of assay conditions for palmitoyl-CoA synthetase and carnitine palmitoyltransferase in homogenates of human blood platelets. Scand J Clin Lab Invest. 1979 Feb;39(1):15–21. doi: 10.3109/00365517909104934. [DOI] [PubMed] [Google Scholar]
  29. Wojtczak L. Effect of long-chain fatty acids and acyl-CoA on mitochondrial permeability, transport, and energy-coupling processes. J Bioenerg Biomembr. 1976 Dec;8(6):293–311. doi: 10.1007/BF00765158. [DOI] [PubMed] [Google Scholar]

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