Skip to main content
. 2024 Nov 5;90:102052. doi: 10.1016/j.molmet.2024.102052

Figure 4.

Figure 4

Suppression of mitochondria-related gene expression in primary Glis3-KO2 RECs and increased expression by exogenous GLIS3. (A) RT-qPCR analysis of the expression of mitochondria-related genes in WT and Glis3-KO2 RECs. Data are represented as mean ± SEM, n ≥ 3. (B) Immunoblot analysis of ATP5A and TFB2M expression in WT and KO primary RECs. (C) Densitometric analysis of ATP5A and TFB2M proteins in WT and KO primary RECs. Data are represented as mean ± SEM, n ≥ 4. (D) Overexpression of GLIS3 in WT and Glis3-KO2 RECs increased Tfb2m and Ppargc1a mRNA expression. RECs were infected with Glis3 lentivirus for 36 h and gene expression analyzed by RT-qPCR. Data are represented as mean ± SEM, n = 3. ∗ Represents p < 0.05.