| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Recombinant anti-TROP2 humanized RS7 antibody | Gilead pharma | Cat #GS-1170562 |
| Goat anti-Human IgG F(ab')2 Secondary Antibody | ThermoFisher | Cat # 31628; RRID: AB_429704 |
| Annexin V-FITC | BioLegend | Cat #640906 |
| Mouse anti-BrdU-FITC conjugate | BioLegend | Cat #364104; RRID: AB_2564481 |
| Mouse anti-H2AX (S139), AF647 conjugate | BioLegend | Cat #613408; RRID: AB_2295046 |
| Goat anti-mouse IgG, AF488 conjugate. | ThermoFisher | Cat # A-11001; RRID: AB_2534069 |
| Donkey anti-Rabbit IgG, AF555 conjugate. | ThermoFisher | Cat #A-21428; RRID: AB_2535849 |
| Goat anti-rat IgG(H+L), AF488 conjugate | ThermoFisher | Cat # A-11006; RRID: AB_2534074 |
| Goat anti-mouse IgG (H+L), AF594 conjugate | ThermoFisher | Cat # A-11005; RRID: AB_2534073 |
| Rabbit anti-TROP2 | Cell Signaling Tech. | Cat #47866; RRID: AB_2938529 |
| Rabbit anti- pCHK1-S296 | Cell Signaling Tech. | Cat #2349; RRID: AB_2080323 |
| Rabbit anti-pCHK1-S345 | Cell Signaling Tech. | Cat #2348; RRID: AB_331212 |
| Mouse anti-CHK1 | Cell Signaling Tech. | Cat #2360; RRID: AB_2080320 |
| Mouse anti-GAPDH | Cell Signaling Tech. | Cat #97166; RRID: AB_2756824 |
| Rabbit anti-βActin | Cell Signaling Tech. | Cat #8457; RRID: AB_10950489 |
| Mouse anti-βActin | Cell Signaling Tech. | Cat #3700; RRID: AB_2242334 |
| Rabbit anti-pCHK2-T68 | Cell Signaling Tech. | Cat #2661; RRID: AB_331479 |
| Rabbit anti-CHK2 | Cell Signaling Tech. | Cat #6334; RRID: AB_11178526 |
| Rabbit anti-γH2AX (S139) | Cell Signaling Tech. | Cat #9718; RRID: AB_2118009 |
| Rabbit anti-pKAP1-S824 | Cell Signaling Tech. | Cat #4127; RRID: AB_2209906 |
| Mouse anti-KAP1 | Cell Signaling Tech. | Cat # 5868; RRID: AB_10707324 |
| Rabbit anti-CyclinB1 | Cell Signaling Tech. | Cat #4138; RRID: AB_2072132 |
| Rabbit anti-ATM | Cell Signaling Tech. | Cat #2873; RRID: AB_2062659 |
| Rabbit anti-Ubiquityl PCNA (L164) | Cell Signaling Tech. | Cat #13439; RRID: AB_2798219 |
| Mouse anti-PCNA | Cell Signaling Tech. | Cat #2586; RRID: AB_2160343 |
| Rabbit anti-RAD51 | Cell Signaling Tech. | Cat #8875; RRID: AB_2721109 |
| Anti-rabbit IgG, HRP-linked | Cell Signaling Tech. | Cat #7074P2; RRID: AB_2099233 |
| Anti-mouse IgG, HRP-linked | Cell signaling Tech. | Cat #7076P2; RRID: AB_330924 |
| Rabbit anti-RPA70, AF488 conjugate | Abcam | Cat #Ab199097 |
| Rat anti-CldU | Abcam | Cat #ab6326; RRID: AB_305426 |
| Rabbit anti-REV1 | ProteinTech. | Cat #17703-1-AP; RRID: AB_3085535 |
| Rabbit anti- MAD2L2/REV7 | ProteinTech | Cat #12683-1-AP; RRID: AB_2139530 |
| Mouse anti-IdU | Novus biological | Cat #NBP2-44056; RRID: AB_3307851 |
| Biological samples | ||
| Patient biopsies | Clinical trial (NCI). | NCT02203513 |
| Chemicals, peptides, and recombinant proteins | ||
| 7-AAD Viability Staining Solution | BioLegend | Cat #420404 |
| Crystal-violet | Sigma-Aldrich | Cat #C0775 |
| 5-Iodo-2'-deoxyuridine (IdU) | Sigma-Aldrich | Cat #I7125 |
| 5-Chloro-2'-deoxyuridine (CldU) | Sigma-Aldrich | Cat #C6891 |
| DAPI nuclear stain | Sigma-Aldrich | Cat #D9542 |
| Propidium iodide | ThermoFisher | Cat #P3566 |
| Prolong glass antifade mountant | ThermoFisher | Cat #P36980 |
| XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) | ThermoFisher | Cat #X6493 |
| RPMI 1640 medium with glutamine | ThermoFisher | Cat #11835055 |
| Restore™ PLUS Western Blot Stripping Buffer | ThermoFisher | Cat #46430 |
| Bromodeoxyuridine (BrdU) | Fisher Scientific | Cat #BDB550891 |
| Berzosertib | Medkoo biosciences | Cat #406258 |
| Sacituzumab govitecan (SG) | Gilead | N/A |
| Control ADC (h679-CL2A-SN-38) | Gilead | N/A |
| D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) | Sigma-Aldrich | Cat #57668 |
| JH-RE-06, TLS inhibitor | Selleckchem | Cat # S8850 |
| Olaparib | Medchem express | Cat # HY-10162 |
| Fetal Bovine serum | BioWest LLC | Cat # S1480 |
| Critical commercial assays | ||
| Ssoadvanced SYBR® Green supermix | BioRad | Cat #1725270 |
| PrimePCR™ SYBR® Green Assay: GAPDH | BioRad | Cat #10025636 |
| iScript™ Advanced cDNA Synthesis Kit | BioRad | Cat #1725037 |
| Deposited data | ||
| RNAseq datasets | Gene expression omnibus (GEO) | GSE278664 |
| Experimental models: Cell lines | ||
| PEO1 | Sigma-Aldrich | #10032308 |
| PEO1-olaJR | Lab stored | Huang et al.29 |
| PEO1-olaR | Lab stored | Huang et al.29 |
| PEO4 | Sigma-Aldrich | #10032309 |
| OVCAR8 | NCI-60 collection | N/A |
| PEO1-Luc | Lab stored | This study |
| PEO1-olaR-Luc | Benjamin Bitler lab | Yamamoto et al.30 |
| PEO1-olaJR-Luc | Lab stored | This study |
| OVCAR3 | American Type Culture Collection (ATCC) | #HTB-161 |
| OVCAR5 | NCI-60 collection | N/A |
| UWB1.289 | American Type Culture Collection (ATCC) | #CRL-2945 |
| TOV21G | American Type Culture Collection (ATCC) | #CRL-3577 |
| SKOV3 | NCI-60 collection | N/A |
| A2780 | Christina Annunziata Lab | Hernandez et al.57 |
| Experimental models: Mice | ||
| NSG mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | NCI inhouse | N/A |
| Recombinant DNA | ||
| pDRGFP, HR assay | Addgene | Cat #26475 |
| pCBASce1, HR assay | Addgene | Cat #26477 |
| Software and algorithms | ||
| GraphPad Prism V. 10.1.1 | Graphpad software LLC | https://www.graphpad.com/ |
| Image Studio | LI-COR instruments | https://www.licor.com |
| Zen Blue | Carl-Zeiss microscopy | https://www.zeiss.com/ |
| Image-J | National Cancer Institute. | https://imagej.net/ij/ |
| Aura image analysis software | Spectral Instruments Imaging LLC. | https://spectralinvivo.com/aura-imaging-software/ |
| FlowJo V. 10.7.2 | BD biosciences | https://www.flowjo.com/ |
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