. 2024 Nov 17;67(22):20438–20454. doi: 10.1021/acs.jmedchem.4c01894

Table 1. Inhibition Data of the Compounds SH7a–t and the Standard Sulfonamide Inhibitor Acetazolamide (AAZ) towards Human CA I, II, IX and XII, as Determined by Using a Stopped-Flow CO₂ Hydrase Assay¹⁸.

Cpd.	R	R₁	K_I (nM)^a
			hCA I	hCA II	hCA IX	hCA XII
SH7a	H	H	51,420	61.3	33.4	42.5
SH7b	H	4-F	48,070	34.5	26.1	51.3
SH7c	H	4-Cl	57,520	65.8	39.8	59.6
SH7d	H	4-OCH₃	54,180	37.6	44.5	22.2
SH7e	H	4-COOEt	63,810	46.2	63.2	27.5
SH7f	H	4-SO₂NH₂	791.7	54.7	24.3	39.3
SH7g	H	thiazol-2-yl	60,790	72.4	67.6	30.4
SH7h	H	4-NO₂	52,270	38.1	37.4	49.7
SH7i	OCH₃	H	73,810	96.9	34.9	35.9
SH7j	OCH₃	4-F	69,580	87.3	22.1	43.4
SH7k	OCH₃	4-Cl	81,340	123.4	17.4	48.8
SH7l	OCH₃	4-OCH₃	76,460	103.7	41.3	16.7
SH7m	OCH₃	4-COOEt	72,770	93.5	55.7	26.9
SH7n	OCH₃	4-SO₂NH₂	837.8	59.3	32.4	32.6
SH7o	OCH₃	thiazol-2-yl	89,140	131.2	35.1	24.3
SH7p	OCH₃	4-Br	85,390	157.1	19.3	57.1
SH7q	OCH₃	4-Br,2-F	83,060	126.3	16.2	65.7
SH7r	OCH₃	3-CF₃	77,690	81.5	23.5	52.4
SH7s	OCH₃	4-Cl,3-CF₃	80,350	114.6	15.9	55.2
SH7t	OCH₃	4-OCF₃	74,170	86.3	29.6	19.0
AAZ			250.0	12.5	26.0	5.7

Mean from three independent assays, measured by using a stopped-flow technique (errors were in the range of ±5–10% of the reported values).

Table 1. Inhibition Data of the Compounds SH7a–t and the Standard Sulfonamide Inhibitor Acetazolamide (AAZ) towards Human CA I, II, IX and XII, as Determined by Using a Stopped-Flow CO2 Hydrase Assay18.