FIG. 4.

Two CTARs independently induce the expression of IRF-7. (A) Schematic diagram of LMP-1 mutants. CTAR-1, CTAR-2, and JAK-3-binding motifs are indicated. (B) Two CTARs independently induce the expression of IRF-7. RPA was performed with IRF-7 and GAPDH probes. Lanes 1, 8, and 12, yeast RNA. Lanes 6 and 7, undigested GAPDH and IRF-7 probes. In all other lanes, RNAs from transfected DG75 cells were used. Lanes 2, 9, and 13, pcDNA-3; lane 3, wild-type (wt) LMP-1; lanes 4, 5, 10, and 14, LMP-PQAA; lane 11, LMPΔCTAR1; lane 15, LMP-IID; and lane 16, LMP-DM. Specific RNA protections are shown. Bottom panel, short-time exposure for GAPDH-protected areas. A representative from three independent experiments is shown. (C) Relative IRF-7 levels from panel B. Data were obtained by normalizing IRF-7 RNA levels to GAPDH RNA levels with the use of a PhosphorImager. The column numbers match the lanes in panel B. (D) Western blot analysis of transfected cells with LMP-1 antibody. The lane numbers match the lanes in panel B.