FIG. 5.

C. elegans HCF interacts with human LZIP in a yeast two-hybrid assay. A yeast GAL1-HIS3 reporter strain was transformed with expression plasmids encoding different HCF proteins fused to the GAL4 DBD together with an expression plasmid encoding either one of two known HCF-1 binding proteins (VP16 and LZIP) or nonbinding proteins (VP16ΔC and SNF-4) fused to a GAL4 AD. (A) Key for GAL4-AD fusion protein samples shown in panel B. (B) Yeast two-hybrid assays. The DBD fusion protein used is indicated above each plate. Successful growth with histidine (+His; left half of each plate) shows that each expression plasmid has no lethal effect. The interaction between a DBD fusion protein and an AD fusion protein is demonstrated by successful growth in the absence of histidine (−His; right half of each plate).