Fig. 2.

EpiCHAOS reflects epigenetic heterogeneity associated with developmental plasticity. A–C Violin plots (left) showing epiCHAOS scores (epiCHAOS) computed in scATAC-seq data from A human hematopoiesis [28], B mouse gastrulation [30], and C drosophila embryogenesis [55]. EpiCHAOS scores were computed per-cell type as annotated in the original publications. Violins represent the scores computed in five random subsamples of 100 cells from each cell type, or once where fewer than 100 cells were available. Plots are ordered by epiCHAOS scores and progenitor cells and undifferentiated tissue types are highlighted in blue. UMAP embeddings (right) illustrating epiCHAOS scores in the same datasets as in violin plots. UMAPs are colored by epiCHAOS scores computed per annotated cell/tissue type. D–F Scatter plots correlating epiCHAOS scores (epiCHAOS) with developmental time as defined by D CytoTRACE score, averaged across cells (human hematopoietic system), E developmental time in days at sample collection, averaged across cells (mouse gastrulation), or F predicted developmental time in hours (Drosophila embryogenesis). EpiCHAOS scores represent the average of pseudo-replicates shown in A–C. Linear regression lines are displayed with Pearson correlation coefficients and p-values