Fig. 3.

EpiCHAOS correlates with features of malignant cell plasticity and aging. A Schematic describing breast and liver cancer datasets used for epiCHAOS calculation. B–C Dot plots illustrate ordered Pearson coefficients after correlation of per-cluster epiCHAOS scores against gene set scores for all Hallmark Gene Ontology biological processes in breast (B) and liver cancer (C). Top 5 correlations in each dataset are highlighted and labeled below. D UMAP embedding (left) of scATAC-seq data from five primary and two metastatic childhood ependymoma tumors [36]. Cells are colored by epiCHAOS scores (epiCHAOS) computed for each cell type. Undifferentiated cells are highlighted in red. Violin plot (right) ordered by epiCHAOS scores (epiCHAOS) for all malignant and non-malignant cell types annotated in ependymoma tumors. Malignant cell types are highlighted in blue. Violins represent the scores computed in five random subsamples of 100 cells from each cell type, or once where fewer than 100 cells were available. E Violin plot ordered by epiCHAOS scores computed in scATAC-seq data from old (blue; n = 3) and young (black; n = 2) mouse HSCs [39]. Violins represent the scores computed in five random subsamples of 100 cells from each group