Fig. 5.

Inhibition of SYK significantly alleviates the central sensitization induced by NTG. a-d Immunoblot identification of the ratio of p-SYK/SYK was performed in the designated groups (n = 6). e-g Immunoblotting of c-fos and CGRP protein levels were identified (n = 6). h, i The intensity of immunoreactivity against CGRP (n = 5). Two-way ANOVA with post-hoc Tukey’s test. R406 efficaciously inhibited the reduction of mechanical thresholds of both basal and post-treatment in hind paw plantar (j, k) and periorbital regions (l, m), while also prolonging the latency for withdrawal to heat (n, o); two-way RM ANOVA with Bonferroni post hoc test. Data are depicted as mean ± SEM; *p < 0.05, ***p < 0.001 compared with the VEH group; ^##p < 0.01 and ^###p < 0.001 compared with the NTG group