Table 1.
Antibody used in Western blotting and immunofluorescence staining
| Antibody | Manufacturer | Catalog number | Host | Dilution |
|---|---|---|---|---|
| For western blot | ||||
| TREM2 | Abcam, UK | ab305103 | Rabbit | 1:1000 |
| c-fos | Santa Cruz, USA | sc166940 | Mouse | 1:500 |
| CGRP | Abcam, UK | ab139264 | Rabbit | 1:1000 |
| SYK | CST, USA | 2712 | Rabbit | 1:1000 |
| p-SYK | CST, USA | 2710 | Rabbit | 1:1000 |
| NLRP3 | CST, USA | 15,101 | Rabbit | 1:1000 |
| ASC | CST, USA | 67,824 | Rabbit | 1:1000 |
| Cleaved-Caspase-1 | Proteintech, China | 22915-1-AP | Rabbit | 1:2000 |
| Mature IL-1β | Abcam, UK | ab234437 | Rabbit | 1:1000 |
| β-actin | Santa Cruz, USA | sc-47,778 | Mouse | 1:1000 |
| HRP conjugated anti-rabbit | Proteintech, China | SA00001-2 | Goat | 1:100000 |
| HRP conjugated anti-mouse | Proteintech, China | SA00001-1 | Goat | 1:100000 |
| For immunofluorescence | ||||
| TREM2 | Abcam, UK | ab305103 | Rabbit | 1:1000 |
| CGRP | Santa Cruz, USA | sc-57,053 | Mouse | 1:100 |
| Iba1 | Abcam, UK | ab5076 | Goat | 1:500 |
| Iba1 | Abcam, UK | ab178846 | Rabbit | 1:500 |
| GFAP | Santa Cruz, USA | sc-33,673 | Mouse | 1;200 |
| NeuN | Abcam, UK | ab104224 | Mouse | 1:1000 |
| Alexa Fluor 488 Donkey anti-mouse IgG | Jackson ImmunoResearch, USA | 715-545-150 | Donkey | 1:200 |
| Alexa Fluor 594 Donkey anti-rabbit IgG | Jackson ImmunoResearch, USA | 711-585-152 | Donkey | 1:500 |
| Alexa Fluor 488 Donkey anti-goat IgG | Jackson ImmunoResearch, USA | 705-545-147 | Donkey | 1:200 |