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. 2024 Mar 14;2:101838. doi: 10.1016/j.gimo.2024.101838

Figure 3.

Figure 3

Functional assay of the Pro905Leu variant of NSD2. A. Schematic representation of Nsd2 variant (knockin) and Nsd2 deletion (knockout) alleles generated by CRISPR/Cas9-mediated genome editing in this study. For the latter, the genomic loci targeted by CRISPR/Cas9 guide RNAs are indicated by arrows. B. Nsd2 p.Pro906Leu knock-in mice showed a decrease in H3K36me2 in thymocytes at E18.5. C. Student’s t test showed significant decrease of H3K36me2 in homozygous mice with P < .01 as indicated ∗. Biological replicates were n = 4. D. Nsd2 p.Pro906Leu homozygous knockin mice showed a decrease in Nsd2 protein. E. Student’s t test showed significant decrease of Nsd2 in homozygous knock-in mice with P < .01 as indicated ∗, (F) but not Nsd2 mRNA, in brains at E18.5 (n = 4).